View and download bd facscanto ii instructions for use manual online. Bd facsdiva software in order for it to be recognized by the computer. Wait 10 seconds, then open the bd facsdiva software and log on. Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Can i use uncompensated acquisition data of my compensation controls for automatic compensation in flowjo. How to operate the bd facscanto flow cytometer preface dear colleague, the bd facscanto flow cytometer is use to operate, however, it is a delicate instrument for measuring fluorescence of single cells. For information on how to operate and maintain your flow cytometer, refer to the instrument users guide. Other software fcs and setup files created within bd facscanto clinical software can be imported into bd facsdiva software v5. Bd facscanto ii quick reference manual pdf download. The bd facsdiva software reference manual assumes you have a working.
See headers and footers on page 100 in the bd facsdiva software reference manual. Bd facscantotm ii users guide for authorization to use the facscanto ii cytometer, you first have to receive a training and must accept the user rules of the facility. Before starting your daily workflow, ensure that your labs software administrator has performed all the necessary tasks to set up the software for your use. In this guide, you will find a description of bd facsdiva software features specific to the hts option. Compensation of a 7 color panel on the bd lsr ii youtube. Interference there are no known incompatibilities with bd facscanto clinical. We offer stateofart analyzing flow cytometers for customer use. Default templates are now provided for certain instrument functions. Aug 25, 2017 this video describes the process of manual and auto compensation in bd facsdiva on the lsr ii. Can i use uncompensated acquisition data of my compensation. The compensation process can be done in an automated or manual manner within the facsdiva software. This guide should help you operating the flow cytometer and leaving it such way that another colleague can use it another day. The automated compensation program is recommended as the software automatically checks the effects of each change in compensation made on all other parameters used something difficult or very time consuming when carrying out the compensation.
Communication between hts unit and software will now be connected. Bd facsdiva software quick reference guide for bd facscanto systems workflow overview the following figure shows the steps for daily workflow using bd facsdiva software. This version runs on the windows 7 32bit operating system. Easily share your publications and get them in front of issuus.
Bd facscanto ii instructions for use manual pdf download. Cytometer can be placed in standby when not running plates, primes, cleans, etc. All other cytometer and fluidics cart functions are controlled by bd facscanto clinical software and bd facsdiva software provided with the instrument. You have remained in right site to begin getting this info. Lei formats or can be exported as tiff or jpg files suitable for analysis with 3rd party image analysis software such as imagepro or imagej. If diva is already running, install hts device and select reinitialize from hts dropdown menu. Follow only those sections that apply to the software you are running.
The bd facsdiva software reference manual assumes you have a working knowledge of basic microsoft windows operation. Setup on page 1 in the bd facsdiva software reference manual. Inspect fluid levels in sheath and waste tanks, replaceempty if needed. Enumeration of peripheral lymphocyte subsets using 6 vs. The loader is controlled by bd facscanto clinical software or bd facsdiva software. Refer to new features in the online help for details.
Biexponential display the biexponential display feature has been enhanced as follows. This manual describes how to use bd facsdiva software. Flowjos default preferences should display the data the same way as seen on the instrument. Bd biosciences bd facscanto ii flow cytometers 422 and 53 configurations with bd facscanto clinical software and bd facsdiva software. The bd facsvia flow cytometry system is an easytouse benchtop solution that helps meet the challenges of rising costs, decreasing reimbursement, laborintensive manual procedures, fewer resources and higher demands for efficiency, so your laboratory runs smoothly and efficiently. Diva is the first native software synth that applies. Bd facsdiva software offers efficient data management tools to simplify data storage as well as improve organization of flow cytometer data. You could purchase lead facscanto ii user guide or get it as soon as feasible. The bd facsdiva software reference manual assumes that you have a working knowledge of basic microsoft windows operation. Bd facscanto clinical software can import worklists from bd facs sample prep assistant spa software v2. To log in to the facsdiva software you need to locate the icon on the desktop and double click it. Launch the divasoftware, log in and start your measurement. Bd facsdiva software v6 with spa iii and lwa sample preppers bd facsdiva software v6.
If you are a new user, use the tutorials to get started with the software. Bd facsdiva software reference manual for version 8. Bd facscanto ii system overview bd facsdiva software v6 bd facsdiva software v6 overview. Bd facsdiva software provides one of the most complete and robust feature sets available for flow cytometry.
Cytometer function is controlled by bd facsdiva software. If you are an experienced user, try the updated tutorials to become familiar with new software features. Ce ivd release 338572 rev a 904 updated for version 4. This guide contains instructions for using bd facsdiva software version 6. Before you begin this guide assumes that you have read the bd facscanto ii instructions for use and the bd facscanto clinical software reference manual, and that you are familiar with cytometer setup. Workflow for facscanto instruments condensed workflow 1. For information on how to operate and maintain your flow cytometer, see your cytometer manual. Quick reference guide,technical documentflow cytometry created date. Introduction this manual explains how to use diva gis.
Sep 23, 2019 i got the bd technical bulletin called standardizing application setup across multiple flow cytometers using bd facsdiva version 6 software which describes how to make two or more machines. Bd facscanto clinical software is supplied with the bd facscanto and facscanto ii systems and includes application panels optimized for use with specific ivd reagent kits. Diva captures the spirit of various analogue synthesizers by letting the user select from a variety of. The bd facsdiva software quick start guide contains tutorials designed to familiarize you with bd facsdiva software. Additional information, including an introductory tutorial, exercises and examples of the use of diva gis can be found on. Alternative procedures, such as lyseandwash or antiigg coated. Analog bd cytometers calibur and scan use the cell quest software line, which produce fcs 2. Firsttime users of bd facsdiva software should read.
Digital bd cytometers facscanto, facslsrii, facsaria, lsrfortessa, etc. Sep 20, 2019 bd facsdiva software quick reference guide for the bd lsr ii. If you are not familiar with the windows operating system, refer to the documentation provided with your computer. Biexponential display is enabled by default, but can be disabled in user preferences, allowing more events to be recorded per experiment. Bd facsdiva software provides a graphic of the cytometers physical optical configuration, showing the layout of detectors, filters, and mirrors. The online help contains all information from the manuals, enhanced with full text search to make it easier to find what you are looking for. If you are not familiar with the windows operating system, see the documentation provided with your computer. Bd facsdiva software quick reference guide for bd facscanto. These panels feature automated gating, calculations, and report generation to deliver consistent, reproducible, and standardized analysis. Bd biosciences facsdiva software features organization.
New worksheet features create headers and footers on the worksheet, including cytometer name, experiment name, user name, date and time, bd facsdiva software version, etc. Compensation controls in the bd facsdiva software reference manual. Biocenter flow cytometry unit is located at the viikki campus and provides flow cytometry analysis services and training for new users. Bd facscanto clinical software c instrument qc, lymphocyte subset tbnk, hlab27, stem cell enumeration bd facsdiva. In the login window, scroll down through the list of user names to. Facscanto ii measuring instruments pdf manual download. Facscanto ii user guide recognizing the way ways to get this book facscanto ii user guide is additionally useful. Page 1 bd facscanto ii flow cytometer quick reference guide this guide contains instructions for both bd facscanto clinical and bd facsdiva software. See the bd facsdiva software reference manual and the bd cytometer setup and tracking application guide for more details. Refer to the bd facsdiva software reference manual for details. Fcs and setup files created within bd facscanto clinical software can be imported into bd facsdiva software v5.
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